ASK1-IN-3

ASK1-IN-3 is a potent and selective ASK1 kinase inhibitor with IC50 of 33.8 nM, as well as inhibits several cell cycle regulating kinases. ASK1-IN-3 has strong HepG2 cancer cells apoptosis induction and potent cell cycle arrest activities[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Research Areas >> Inflammation/Immunology

Signaling Pathways >> MAPK/ERK Pathway >> MAP3K

IC50: 33.8 nM (ASK1)[1]

ASK1-IN-3 (compound 14l) (10, 20 and 50 μM; 48 hours) induces PARP cleavage in a dose dependent manner, which indicates the induction of HepG2 cells apoptosis[1]. ASK1-IN-3 (1-16 μM; 24 hours) significantly arrests cycle progression at G1 phase[1]. Apoptosis Analysis Cell Line: HepG2[1] Concentration: 10, 20 and 50 μM Incubation Time: 48 hours Result: Induced PARP cleavage in a dose dependent manner. Cell Cycle Analysis Cell Line: HepG2[1] Concentration: 1, 2, 4, 8, and 16 μM Incubation Time: 24 hours Result: Significantly arrested cycle progression at G1 phase.

[1]. Zhang S, Huang C, Lyu X, et al. Discovery of a 2-pyridinyl urea-containing compound YD57 as a potent inhibitor of apoptosis signal-regulating kinase 1 (ASK1). Eur J Med Chem. 2020;195:112277.