Apoptosis inducer 4

Apoptosis inducer 4 (Compound 12b) is an apoptosis inducer with anticancer activities[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Apoptosis inducer 4 (Compound 12b) (0-50 µM, 72 h) shows antiproliferative activities against cancer cells without obvious cytotoxicity against normal cells[1]. Apoptosis inducer 4 (0-2 µM, 48 h) causes cell cycle arrest at S phase in K562 cells and G1 phase in HepG2 cells[1]. Apoptosis inducer 4 (0-4 µM, 24 and 48 h) induces pyknosis in K562 cells, and induces mitochondrial membrane potentials decline[1]. Apoptosis inducer 4 (0-2 µM, 72 h) induces apoptosis in K562 and HepG2 cells[1]. Cell Proliferation Assay[1] Cell Line: HepG2, MCF-7, HCT-116, B16, K562, L-02 and PBMC Concentration: 0-50 µM Incubation Time: 72 h Result: Showed antiproliferative activities with IC50 values of 2.57 ± 0.12, 16.15 ± 1.02, 5.81 ± 1.17, 12.94 ± 0.88, 0.95 ± 0.06, 17.59 ± 0.93 and >50 µM against HepG2, MCF-7, HCT-116, B16, K562, L-02 and PBMC cells, respectively. Cell Cycle Analysis[1] Cell Line: K562 and HepG2 cells Concentration: K562 and HepG2 cells Incubation Time: 48 h Result: Arrested K562 cell cycle at S phase and HepG2 cell at G1 phase. Apoptosis Analysis[1] Cell Line: K562 and HepG2 cells Concentration: K562 and HepG2 cells Incubation Time: 72 h Result: Apoptotic ratios of K562 and HepG2 cells obviously increased in a concentration-dependent manner.

[1]. Li H, et al. Hydrogen sulfide releasing oridonin derivatives induce apoptosis through extrinsic and intrinsic pathways. Eur J Med Chem. 2020 Feb 1;187:111978.