CPUL1

CPUL1 is a TrxR inhibitor, which shows proliferation-inhibitory and anti-metastatic activity against A549 cells. CPUL1 influences EMT (epithelial-mesenchymal transition) via inducing ROS-mediated ERK/JNK signaling by inhibiting TrxR1 enzyme activity. CPUL1 in combination with α-Lipoic Acid (HY-N0492) or Dithiodipropionic acid (HY-W014395) is more effective[1].

Research Areas >> Cancer

Signaling Pathways >> Others >> Others

CPUL1 (2.5, 5, 10, 20, 40 μM; 48 h) inhibits A549 cell proliferation and (2, 4, 8 μM; 48 h) colony formation[1]. CPUL1 (4, 8 μM; 0, 24, 48 h) inhibits A549 cells migration and (2, 4, 8 μM; 48 h) invasion[1]. CPUL1 hinders EMT (epithelial-mesenchymal transition) progress and affects MAPK pathway in A549 cells[1]. Cell Proliferation Assay[1] Cell Line: A549 cells Concentration: 2.5, 5, 10, 20, 40 μM Incubation Time: 48 h Result: Showed a dose-dependent cytotoxicity, with an IC50 value of 7.61 μM. Cell Viability Assay[1] Cell Line: A549 cells Concentration: 2, 4, 8 μM Incubation Time: 48 h Result: Significantly abolished the capacity of A5459 cells to form colonies at the dose-dependent concentration. Cell Migration Assay [1] Cell Line: A549 cells Concentration: 4, 8 μM Incubation Time: 0, 24, 48 h Result: Inhibited the migration of A549 cells in a dose- and time-dependent manner.