lucidone

Lucidone, an anti-inflammatory agent that can be isolated from the fruit of Lindera erythrocarpa Makino. Lucidone inhibits LPS-induced NO and PGE2 production in RAW 264.7 mouse macrophages. Lucidone also decreases TNF-α secretion, iNOS and COX-2 expression. Lucidone prevents NF-κB translocation and inhibits JNK and p38MAPK signals. Lucidone also has inhibitory activity against Dengue virus (DENV) (EC50=25 μM)[1][2].

Signaling Pathways >> NF-κB >> IKK

Research Areas >> Infection

Signaling Pathways >> Immunology/Inflammation >> NO Synthase

Signaling Pathways >> MAPK/ERK Pathway >> p38 MAPK

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Immunology/Inflammation >> COX

Signaling Pathways >> MAPK/ERK Pathway >> JNK

p38 MAPK

JNK

p50

p65

COX-2

NF-κB

iNOS

IKK

Lucidone (10 μg/mL, 25 μg/mL; 1 h 或 20 h) 降低 RAW 264.7 巨噬细胞受 LPS (1 μg/mL) 诱导而导致 TNF-α 分泌 (20 h) 以及 PGE2 产量 (1 h) 的增加[1]。 Lucidone (10 μg/mL, 25 μg/mL; 8 h) 抑制 RAW 264.7 巨噬细胞受 LPS (1 μg/mL) 诱导而导致的 iNOS 和 COX-2 表达的增加[1]。 Lucidone (10 μg/mL, 25 μg/mL; 1 h) 抑制 RAW 264.7 巨噬细胞受 LPS (1 μg/mL) 诱导而导致的 NF-κB p65/p50 水平的增加，以及 IKK 蛋白的磷酸化[1]。 Lucidone (0-40 μM; 3 d) 降低感染 Huh-7 细胞中 DENV 的病毒滴度[2]。 Western Blot Analysis[1] Cell Line: RAW 264.7 macrophages Concentration: 5 μg/mL, 10 μg/mL, 25 μg/mL Incubation Time: 1 hour Result: Inhibited LPS-induced NF-κB nuclear translocation, decreased the level of p65 and of the p50 subunits at 10 μg/mL and 25 μg/mL. Inhibited the phosphorylation of IKK at 10 μg/mL and 25 μg/mL. Cell Viability Assay[2] Cell Line: Huh-7 cells infected with DENV Concentration: 5 μM, 10 μM, 20 μM, and 40 μM Incubation Time: 3 days Result: Showed no significant cell viability on Huh-7 cells, while leading to a decrease on viral load of DENV.

Lucidone (1 mg/kg; 颅内注射; 一周内 3 次) 显著提高 DENV 感染小鼠的存活率和体重[2]。 Animal Model: DENV-infected ICR mice[2] Dosage: 0.4 mg/kg, 1 mg/kg Administration: Intracranial injection; 3 times dose at day 2, 4, 6 after DENV infection Result: Increased the survival rate and body weight of DENV-infected mice on day 7.

[1]. Senthil Kumar KJ, et aL. Lucidone inhibits iNOS and COX-2 expression in LPS-induced RAW 264.7 murine macrophage cells via NF-kappaB and MAPKs signaling pathways. Planta Med. 2009 Apr;75(5):494-500.  

[2]. Chen WC, et al. Lucidone suppresses dengue viral replication through the induction of heme oxygenase-1. Virulence. 2018 Jan 1;9(1):588-603.