NL-1
Names
[ CAS No. ]:
188532-26-5
[ Name ]:
NL-1
Biological Activity
[Description]:
NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively. NL-1-mediated death in leukemic cells requires the activation of the autophagic pathway[1].
[Related Catalog]:
[Target]
MitoNEET[1]
[In Vitro]
NL-1 (10-100 μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with IC50s of 29.48 µM, 94.26 µM, respectively. TOM1, BV173, NALM1 and JM1 all have similar IC50 values of around 60 µM for NL-1[1]. NL-1 (60 μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death[1]. NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells[1]. Cell Proliferation Assay[1] Cell Line: REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines Concentration: 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 100 μM Incubation Time: 72 hours Result: Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. Cell Autophagy Assay[1] Cell Line: REH, REH/Ara-C cell lines Concentration: 60 μM Incubation Time: 6 hours Result: Induced cell autophagy.
[In Vivo]
NL-1 (10 mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model[1]. Animal Model: 10 female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells[1] Dosage: 10 mg/kg Administration: Intraperitoneal injection; daily; for 5 days Result: Showed antileukemic activity in an in vivo mouse ALL model.
[References]
Chemical & Physical Properties
[ Molecular Formula ]:
C18H25NO3S
[ Molecular Weight ]:
335.46100
[ Exact Mass ]:
335.15600
[ PSA ]:
91.70000
[ LogP ]:
4.21010
Safety Information
[ Hazard Codes ]:
Xi