3,3'-Diaminobenzidine tetra hydrochloride 2-hydrate

Names

[ Name ]:
3,3'-Diaminobenzidine tetra hydrochloride 2-hydrate

[Synonym ]:
MFCD00150106
UNII-Z3KH58591V
3,3',4,4'-Biphenyltetramine tetrahydrochloride dihydrate
EINECS 231-018-9
3,3'-Diaminobenzidine tetrahydrochloride hydrate
[1,1'-Biphenyl]-3,3',4,4'-tetramine, hydrochloride, hydrate (1:4:2)

Biological Activity

[Description]:

3,3-Diaminobenzidine tetrahydrochloride dehydrate is a staining agent that is often used in histochemical (HC) and immunohistochemical (IHC) procedures. 3,3-Diaminobenzidine tetrahydrochloride dehydrate can be used for peroxisomal staining and mitochondrial cytochrome c oxidase activity staining[1].

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

[In Vitro]

作为一种染料，3,3-Diaminobenzidine (DAB) tetrahydrochloride dehydrate 被氧化并转化为一种不溶性聚合物，在反应部位沉淀为深棕色色素，使目标分子可见。在免疫组化中，3,3-Diaminobenzidine tetrahydrochloride dehydrate 作为过氧化物酶的底物偶联到一抗或二抗。使用 3,3-Diaminobenzidine tetrahydrochloride dehydrate 的组织化学染色方法包括细胞器中过氧化物酶体染色和线粒体细胞色素 c 氧化酶 (COX，也称为呼吸复合体 IV) 活性染色[1]。 Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 对于细胞色素 c 氧化酶 (COX) 组织化学染色 1. 用 ~50µL COX 染色液 (0.1 M NaPO4, 4 mM 3,3-diaminobenzidine (DAB) tetrahydrochloride dehydrate, 100 µM cytochrome c, 2 mg/mL catalase) 在 37°C 下孵育切片 45 分钟。 2. 在 PBS 中清洗 3 次，每次 5 分钟，用分级乙醇 (75% 和 95% 各 1 分钟，100% 乙醇 10 分钟) 脱水。 3. 染色后，样品完全干燥后取样。

[References]

[1]. Dölle C, et al. 3,3'-Diaminobenzidine staining interferes with PCR-based DNA analysis. Sci Rep. 2018 Jan 19;8(1):1272.

Chemical & Physical Properties

[ Boiling Point ]:
481.7ºC at 760 mmHg

[ Melting Point ]:
280 °C

[ Molecular Formula ]:
C₁₂H₂₂Cl₄N₄O₂

[ Molecular Weight ]:
396.141

[ Flash Point ]:
282.7ºC

[ Exact Mass ]:
394.049683

[ PSA ]:
122.54000

[ LogP ]:
7.08660

[ Vapour Pressure ]:
1.95E-09mmHg at 25°C

[ Storage condition ]:
2~8℃

Safety Information

[ Hazard Codes ]:
Xn:Harmful;

[ Risk Phrases ]:
R22;R36/37/38;R40

[ Safety Phrases ]:
26-36/37/39-45

[ WGK Germany ]:
3

[ Hazard Class ]:
9.0