Lenercept

Lenercept (Ro 45-2081) is a recombinant fusion protein that consists of the soluble TNF-receptor (p55) linked to the Fc portion of human IgG1[1].

Research Areas >> Inflammation/Immunology

TNFR[1]

Lenercept (TNFR-IgG) 在放线菌素 D (HY-17559) 处理的小鼠 L-M 细胞中阻断 TNF-α 和 TNF-β 的细胞溶解作用，IC50 分别为 0.5 μg/mL 和 1.5 μg/mL[2]。

Lenercept (Ro 45-2081) 抑制 Sephadex 诱导的大鼠肺损伤[1]。 Lenercept (TNFR-IgG; 0.8-20 μg/mouse; i.v.; once) 当内毒素激发之前或之后不久给药时，可以预防或显著延迟内毒素诱导的小鼠致死率[2]。 Animal Model: Male Sprague-Dawley rats[1] Dosage: 1 and 3 mg/kg Administration: Intraperitoneal injection, 1 h before administration of Sephadex for the 24 h study or 1 h before and at 24 and 48 h after Sephadex for the 72 h study Result: Inhibited the neutrophilia at 24 h after Sephadex. At 72 h after Sephadex, significantly reduced the neutrophil influx into bronchoalveolar lavage fluid (BALF) but had no inhibitory effect on eosinophil number. Animal Model: 6- to 8-week-old female BALB/c mice, septic shock model[2] Dosage: 0.8, 4 or 20 μg/mouse Administration: IV, single dose Result: Injection 0.5 h prior to Salmonella abortus-derived endotoxin (LD100 dose) administration prevented lethality at a dose of 20 μg per mouse and provided partial protection at lower doses. Injection of 10 μg per mouse provided significant protection 0.5 h before, 0.5 h after, or 1 h after endotoxin injection but little protection 2 h after endotoxin injection.

[1]. Gater PR, et al. Inhibition of Sephadex-induced lung injury in the rat by Ro 45-2081, a tumor necrosis factor receptor fusion protein. Am J Respir Cell Mol Biol. 1996 May;14(5):454-60.  

[2]. Ashkenazi A, et al. Protection against endotoxic shock by a tumor necrosis factor receptor immunoadhesin. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10535-9.