Cimiside E

Cimiside E (25-Anhydrocimigenol xyloside) is a triterpene xyloside, Cimiside E possesses apoptotic action on gastric cancer cells, with an IC50 value of 14.58 μM. Cimiside E induces cell cycle arrest at G2/M phase, and mediates apoptosis through the induction of the Caspase cascade for both the extrinsic and intrinsic pathways[1][2].

Research Areas >> Cancer

Signaling Pathways >> Apoptosis >> Caspase

Caspase 3

Cimiside E (30-90 μM; 24 h) 阻滞细胞周期，诱导 ASG 细胞凋亡[1]。 Cimiside E (30-90 μM; 12-48 h) 对 AGS 细胞有较强的细胞毒性，并表现出抗增殖活性[1]。 Cimiside E (15-60 μM; 6-24 h) 诱导 ASG细胞中 DNA 片段化，(30-60 μM; 1-6 h) 并在 3 h 时激活 FasL 的表达，在 1 h 激活 Fas[1]。 Cimiside E (30-90 μM; 3-24 h) 介导 caspase 级联，增加 Bax/Bcl-2 比值，降低突变型 p53 和 procaspase 3 蛋白水平[1]。 Western Blot Analysis[1] Cell Line: Cimiside E. AGS cells Concentration: 30 μM, 60 μM, and 90 μM Incubation Time: 3 h, 6 h, 12 h, and 24 h Result: Increased the ratio of Bax/Bcl-2 expression from 60 μM. Decreased mutant type (mt) p53 levle from 12 h at 30 µM. Suppressed the protein level of procaspase 3 in a dose-dependent manner from 30 μM. Cell Proliferation Assay[1] Cell Line: Cimiside E. AGS cells Concentration: 30 μM, 60 μM, and 90 μM Incubation Time: 12 h, 24 h, and 48 h Result: Inhibited ASG cells proliferation with IC50s of 28.7, 14.6 and 8.1 µM, respectively, for 30 μM, 60 μM, and 90 μM treatment. Cell Cycle Analysis[1] Cell Line: Cimiside E. AGS cells Concentration: 30 μM, 60 μM, and 90 μM Incubation Time: 3 h, 6 h, and 24 h Result: Induced cell cycle arrest at S phase in a low concentration (30 μM), but arrested cell cycle at G2/M phase in higher concentration (60 μM and 90 μM).

[1]. Guo LY, et al. Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells. Arch Pharm Res. 2009 Oct;32(10):1385-92.  

[2]. Jamróz MK, et al. One new and six known triterpene xylosides from Cimicifuga racemosa: FT-IR, Raman and NMR studies and DFT calculations. Spectrochim Acta A Mol Biomol Spectrosc. 2012 Jul;93:10-8.