5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM

Names

[ Name ]:
5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM

[Synonym ]:
Guanosine, 5'-O-[hydroxy[[hydroxy(phosphonoamino)phosphinyl]oxy]phosphinyl]-, sodium salt (1:3)
Trisodium 5'-O-[({[(hydroxyphosphinato)amino]phosphinato}oxy)phosphinato]guanosine

Biological Activity

[Description]:

Guanylyl Imidodiphosphate (Guanosine 5'-[β,γ-imido]triphosphate) trisodium is a non-hydrolysable GTP analogue, an activator of ADP-ribosylation factor (ARF) and a potent stimulator of adenylate cyclase. Imidodiphosphate trisodium can be used in protein synthesis studies[1][2][3].

[Related Catalog]:

Research Areas >> Endocrinology

Signaling Pathways >> GPCR/G Protein >> Adenylate Cyclase

[In Vitro]

Guanylyl Imidodiphosphate trisodium (GppNHp) (100 μM) induces the secretion of dense granules from permeabilized platelets in response to calcium ion stimulation, and GTP-Ral enhances the calcium sensitivity of permeabilized platelet dense granule secretion which is inhibited by Sec5-RBD[1]. Guanylyl Imidodiphosphate trisodium (GppNHp) (0.017-333 μM) causes a significant reduction in the lag time required to stimulate adenylate cyclase activity[3].

[References]

[1]. Mitsunori Kawato, et al. Regulation of platelet dense granule secretion by the Ral GTPase-exocyst pathway. J Biol Chem. 2008 Jan 4;283(1):166-174.

[2]. J Goldberg, et al. Structural basis for activation of ARF GTPase: mechanisms of guanine nucleotide exchange and GTP-myristoyl switching. Cell. 1998 Oct 16;95(2):237-48.

[3]. C Gaudin, et al. Overexpression of Gs alpha protein in the hearts of transgenic mice. J Clin Invest. 1995 Apr;95(4):1676-83.

Chemical & Physical Properties

[ Melting Point ]:
230 °C

[ Molecular Formula ]:
C₁₀H₁₄N₆Na₃O₁₃P₃

[ Molecular Weight ]:
588.141

[ Exact Mass ]:
587.952454

[ Storage condition ]:
20°C

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
36/37/38

[ Safety Phrases ]:
26-36/37

[ RIDADR ]:
NONH for all modes of transport

Articles

Insights from reconstitution reactions of COPII vesicle formation using pure components and low mechanical perturbation.

Biol. Chem. 395(7-8) , 801-12, (2014)

As shape transformations of membranes are vital for intracellular trafficking, it is crucial to understand both the mechanics and the biochemistry of these processes. The interplay of these two factor...

G proteins.

Trends Biochem. Sci. 17 , 383-387, (1992)

The family of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) serves an essential role in transducing receptor-generated signals across the plasma membrane. Recent findings ...

Requirement of GTP hydrolysis for dissociation of the signal recognition particle from its receptor.

Science 252 , 1171-1173, (1991)

The signal recognition particle (SRP) directs signal sequence specific targeting of ribosomes to the rough endoplasmic reticulum. Displacement of the SRP from the signal sequence of a nascent polypept...

Related Compounds

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