Astrophloxine

Names

[ Name ]:
Astrophloxine

[Synonym ]:
1,1'-Diethyl-3,3,3',3'-tetramethylindocarbocyanine Iodide
diic2(3)
1,3-Bis-(1-aethyl-3,3-dimethyl-3H-indol-2-yl)-trimethinium,Jodid
1,3-bis-(1-ethyl-3,3-dimethyl-3H-indol-2-yl)-trimethinium,iodide
Astrophloxine
N,N'-Diethyl-3,3'-tetramethylindocarbocyanine iodide
Astrophloxin
DILC2(3)
1-Ethyl-2-[3-(1-ethyl-1,3-dihydro-3,3-diMethyl-2H-indol-2-ylidene)-1-propen-1-yl]-3,3-diMethyl-3H-indoliuMIodide
SNC 2

Biological Activity

[Description]:

Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[Target]

Antiparallel dimer[1]

[In Vitro]

Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers[1]. Protocol for CSF analyses[1]: 1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2). 2.Dilute Astrophloxine to 0.5 μM with binding buffer. 3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3. Detect at λex=540 nm/λem=570 nm.

[In Vivo]

Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue[1].

[References]

[1]. Lee JC, et al. Discovery of Chemicals to Either Clear or Indicate Amyloid Aggregates by Targeting Memory-Impairing Anti-Parallel Aβ Dimers. Angew Chem Int Ed Engl. 2020 Jul 6;59(28):11491-11500.

Chemical & Physical Properties

[ Molecular Formula ]:
C₂₇H₃₃IN₂

[ Molecular Weight ]:
512.46900

[ Exact Mass ]:
512.16900

[ PSA ]:
6.25000

[ LogP ]:
3.36830

MSDS

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Safety Information

[ Hazard Codes ]:
Xi

Synthetic Route

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Precursor & DownStream

Precursor

DownStream

Related Compounds

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