Cobalt protoporphyrin IX

Cobalt protoporphyrin IX is a potent and specific heme oxygenase-1 (HO-1) inducer. Cobalt protoporphyrin IX exhibits broad-spectrum antiviral activities against Influenza A virus (IAV)[1].

Signaling Pathways >> Anti-infection >> Influenza Virus

Research Areas >> Infection

Cobalt protoporphyrin IX (72 h) 对 MDCK 细胞和 RAW264.7 细胞的最大无毒浓度 (TC0) 均为 3.13 μM[1]。 Cobalt protoporphyrin IX (2 μM; 0-24 h) 在 MDCK 细胞和 RAW264.7 细胞中以时间依赖的方式增加 HO-1 细胞内蛋白水平[1]。 Cobalt protoporphyrin IX 对 Empty Cell A/FortMonmouth /1/1947、A/TianjinJinnan /15/2009、A/Wuhan /359/1995、A/FujianTongan /196/2009 和 BY/FujianXinluo /54/2006 流感病毒的 IC50分别为 0.40 ± 0.16、0.42 ± 0.15、0.46 ± 0.19、0.34 ± 0.05 和 0.64 ± 0.30 μM[1]。 Cobalt protoporphyrin IX (0.25-2 μM; 18 or 24 h) 通过增强 IFN 反应抑制 IAV 复制，抗 IAV 活性不依赖于 HO-1 的催化功能[1]。 Cobalt protoporphyrin IX (2 μM; 0-6 h) 诱导 HO-1 抗 IAV 活性可能依赖于 IRF3 磷酸化和易位，以增强抗病毒 IFN 应答[1]。 Western Blot Analysis[1] Cell Line: MDCK cells and RAW264.7 cells Concentration: 2 μM Incubation Time: 0, 3, 6, 12 and 24 h Result: Significantly increased the intracellular protein levels of HO-1 in a time-dependent manner in MDCK cells and RAW264.7 cells. Real Time qPCR[1] Cell Line: RAW264.7 cells were infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI) Concentration: 0.25, 0.5, 1 and 2 μM Incubation Time: 18 h Result: Enhanced mRNA expressions of IFN-α/β, as well as protein expressions of some ISGs, such as IFN-inducible transmembrane protein 3 (IFITM3), double-stranded RNA-dependent protein kinase (PKR) and 2′-5′-oligoadenylate synthetase 1 (OAS1), in a dose-dependent manner. Western Blot Analysis[1] Cell Line: RAW264.7 cells or RAW264.7 cells infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI) Concentration: 2 μM Incubation Time: 0, 2, 4 and 6 h Result: Increased protein levels of IRF3 and p-IRF3. Promoted the cytoplasmic protein levels of IRF3 and p-IRF3 in RAW264.7 cells following the enhancement of HO-1 protein expression after 6 h. Induced nuclear IRF3 and nuclear p-IRF3 accumulation within 6 h of treatment.

[1]. Ma LL, et al. heme oxygenase-1 agonist CoPP suppresses influenza virus replication through IRF3-mediated generation of IFN-α/β. Virology. 2019 Feb;528:80-88.