Mechanism??Based Inactivation of Coenzyme B12??Dependent 2??Methyleneglutarate Mutase by (Z)??Glutaconate and Buta??1, 3??diene??2, 3??dicarboxylate

W Buckel, AJ Pierik, S Plett, A Alhapel…

Index: Buckel, Wolfgang; Pierik, Antonio J.; Plett, Sandra; Alhapel, Ashraf; Suarez, Diana; Tu, Shang-Min; Golding, Bernard T. European Journal of Inorganic Chemistry, 2006 , # 18 p. 3622 - 3626

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Citation Number: 4

Abstract

Abstract In the presence of holo 2-methyleneglutarate mutase, buta-1, 3-diene-2, 3- dicarboxylate and (Z)-glutaconate [(Z)-pent-2-ene-1, 5-dicarboxylate], but not (E)- glutaconate, each induced homolysis of the Co–C bond of coenzyme B 12 to afford cob (II) alamin and the 5′-deoxyadenosyl radical. The latter probably added to the double bond in (Z)-glutaconate and one of the double bonds in buta-1, 3-diene-2, 3-dicarboxylate to ...