Bioorganic & medicinal chemistry

A colormetric assay for catechol-O-methyltransferase

K Bailey, R Cowling, EW Tan, D Webb

Index: Bailey, Karl; Cowling, Rebecca; Tan, Eng Wui; Webb, Daniel Bioorganic and Medicinal Chemistry, 2004 , vol. 12, # 3 p. 595 - 601

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Citation Number: 4

Abstract

A series of catechol diazo dyes were synthesized and tested as substrates for the enzyme catechol-O-methyltransferase (COMT) with the aim of developing a sensitive HPLC assay method using visible wavelength light detection. A method was developed which allowed for the determination of the two regioisomeric methylated products of the COMT catalyzed reaction of 4-[(3, 4-dihydroxyphenyl) azo] benzenesulfonate with S-adenosylmethionine ( ...