[Determination of quercetin-3-O-alpha-L-arabinopyranoside in Periploca forrestii by RP-HPLC].

Huaguo Chen, Xin Zhou, Guihong Cao, Xiuhai Gan

Index: Zhongguo Zhong Yao Za Zhi 35(10) , 1284-6, (2010)

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Abstract

The aim of the paper was to develop a HPLC method for the quality control of Periploca forrestii Schltr. The 18 samples were analyzed on a Hypersil C18 column. The mobile phase was methanol-water (33:67) and the flow rate was 1 mL x min(-1). The detection wavelength was at 370 nm and column temperature was 25 degrees C. The linear relationship was good (r = 0.999 9) in the range of 0.204 4-2.044 microg for quercetin-3-O-alpha-L-arabinopyranoside. The average recovery was 97.78% (RSD 0.8%, n = 9). The contents of 18 samples varied from 0.171% to 0.264%. The method showed high precision, good repeatability and stability, so it can be used to assess the quality of P. forrestii.