Enzyme studies on human and snail beta-mannosidase using a fluorescence assay and an HPLC/diode array method with Man beta (1-4)GlcNAc as substrate.
J van Pelt, C H Hokke, L Dorland, M Duran
Index: Int. J. Biochem. 24(4) , 669-73, (1992)
Full Text: HTML
Abstract
1. Snail beta-mannosidase showed a Km value of 0.05 mM toward MU-beta-Man and could not be inhibited by Man, GlcNAc, Man beta(1-4)GlcNAc, Man beta(1-4)GlcNAc beta(1-N)urea or Man beta(1-4) GlcNAc beta(1-4)GlcNAc. 2. The Km value of the snail enzyme towards Man beta(1-4)GlcNAc, as measured by HPLC, was 10 mM, explaining the lack of inhibition. 3. The Km value of the human serum beta-mannosidase towards MU-beta-Man was 0.3 mM, but the human enzyme was not capable of degrading Man beta(1-4)GlcNAc in detectable amounts.
Related Compounds
Related Articles:
1980-01-01
[Eur. J. Biochem. 103(2) , 307-12, (1980)]
Kinetics of interaction of some alpha- and beta-D-monosaccharides with concanavalin A.
1980-09-01
[Biochim. Biophys. Acta 631(3) , 428-38, (1980)]
1991-04-01
[Zentralbl. Bakteriol. 275(1) , 118-22, (1991)]
Binding of methylumbelliferyl mannoside to concanavalin A under high pressure.
1984-10-09
[Biochim. Biophys. Acta 790(1) , 87-90, (1984)]
The soluble alpha-mannosidases of Drosophila melanogaster.
1997-07-01
[Insect Biochem. Mol. Biol. 27(7) , 657-61, (1997)]