Cloning and sequence analysis of two laccase complementary DNAs from the ligninolytic basidiomycete Trametes versicolor.
E Ong, W B Pollock, M Smith
Index: Gene 196(1-2) , 113-9, (1997)
Full Text: HTML
Abstract
Laccases are oxidoreductase enzymes involved in the oxidation of various phenolic compounds. They may play a role in the biodegradation of lignin and in the dechlorination of chlorophenols. The cDNAs encoding laccase LccI and a putative laccase LccIV and the gene for LccI from the white-rot basidiomycete Trametes versicolor were cloned, sequenced and characterized. The genomic DNA of lccI consists of 2128 bp, with the coding region interrupted by 10 introns; the cDNA consists of a 1560 bp open reading frame (ORF). The cDNA of the putative lccIV gene consists of a 1581 bp ORF, with a 794 bp 5' untranslated region. The size of the major transcript for both lccI and lccIV is approximately 2.3 kb. Transcription of lccIV was induced by 2,5-dimethylaniline, whereas the opposite effect was observed for lccI. Laccases I and IV contain highly conserved histidinyl and cysteinyl residues, believed to be involved in binding copper, and share extensive sequence similarity with other laccases produced by both ligninolytic and non-ligninolytic fungi.
Related Compounds
Related Articles:
2009-01-01
[J. Chromatogr. A. 1216(41) , 6970-3, (2009)]
Determination method for xylidines in workplace air.
2011-01-01
[J. Occup. Health 53(3) , 230-3, (2011)]
Oxidation of 2, 5-dimethylaniline in NH 4 F· 2.35 HF medium Characteristics of the resulting conducting polymer. Genies EM and Noel P.
[J. Electroanal. Chem. Interfac. Electrochem. 296(2) , 473-490, (1990)]
Synthesis and characterization of corrosion protective poly (2, 5-dimethylaniline) coatings on copper. Shinde V, et al.
[Appl. Surf. Sci. 253(3) , 1037-1045, (2006)]
Poly (2, 5-dimethylaniline)-MWNTs nanocomposite: a new material for conductometric acid vapours sensor. Bavastrello V, et al.
[Sens. Actuators B Chem. 98(2) , 247-253, (2004)]