Preparation, application and testing of permanent antibacterial and antiviral coatings.

Jayanta Haldar, Alisha K Weight, Alexander M Klibanov

Index: Nat. Protoc. 2 , 2412, (2007)

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Abstract

Protocols for the synthesis of the microbicidal polycation N,N-dodecyl,methyl-polyethylenimine and coating (painting) of glass slides with this polycation's butanol solution are described. Subsequently detailed are the procedures for validating that the resultant coated slides are essentially 100% lethal to the human bacterial pathogens, Staphylococcus aureus and Escherichia coli, as well as to two common strains of influenza virus. The time required to prepare and apply the cationic polymer and to test its microbicidal efficiency is conservatively estimated to be <4 weeks.