Therapeutic Drug Monitoring 1992-10-01

High-performance liquid chromatographic analysis of cyclosporin G (Nva2-cyclosporine) in human blood.

T M Annesley, K Matz, A B Leichtman

Index: Ther. Drug Monit. 14(5) , 397-401, (1992)

Full Text: HTML

Abstract

A sensitive high-performance liquid chromatographic method for the analysis of the immunosuppressant cyclosporin G (OG 37-325, Nva2-cyclosporine, CsG) in whole blood has been developed. Sample preparation, employing cyclosporin A (CsA) as internal standard, involves organic extraction with methyl t-butyl ether under sequential acidic and basic conditions. Chromatography is performed using a 2 mm inside diameter x 25 cm column packed with 5 microns octyl (C8) material. An isocratic mobile phase comprised of acetonitrile:methanol:water, at a flow rate of 0.4 ml/min, is utilized. Separation is monitored at 230 nm. Data are also presented that demonstrate the use of CsG as an alternative internal standard to cyclosporin D for liquid chromatographic determinations of CsA.

Related Compounds

Related Articles:

Antimalarial activity of cyclosporins A, C and D.

1994-03-01

[Afr. J. Med. Med. Sci. 23(1) , 47-51, (1994)]

Enzymatic synthesis of cyclosporin A.

1987-12-25

[J. Biol. Chem. 262(36) , 17258-9, (1987)]

In vitro and in vivo comparative studies on immunosuppressive properties of cyclosporines A, C, D and metabolites M1, M17 and M21.

1993-01-01

[Immunopharmacol. Immunotoxicol. 15(2-3) , 163-77, (1993)]

Does the binding of cyclosporine to calmodulin result in immunosuppression?

1986-10-03

[Science 234(4772) , 68-71, (1986)]

The weak immunosuppressant cyclosporine D as well as the immunologically inactive cyclosporine H are potent inhibitors in vivo of phorbol ester TPA-induced biological effects in mouse skin and of Ca2+/calmodulin dependent EF-2 phosphorylation in vitro.

1988-01-29

[Biochem. Biophys. Res. Commun. 150(2) , 545-51, (1988)]

More Articles...