New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore.
T O Sippel
Index: J. Histochem. Cytochem. 29(2) , 314-6, (1981)
Full Text: HTML
Abstract
N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM) and the corresponding iodoacetamide are described. When applied to sections at pH 6 and 9, respectively, the two fluorogens are very similar in their reactivity and selectivity toward thiols with which they form adducts having much the same bright blue fluorescence. However, difference in other properties favor CPM for further study.
Related Compounds
Related Articles:
[J. Chromatogr. A. 530(1) , 57-64, (1990)]
1992-03-03
[Biochemistry 31(8) , 2368-75, (1992)]
Self-association of Ca(2+)-binding peptides induced by lanthanide ions: a fluorescence study.
1993-09-01
[Anal. Biochem. 213(2) , 296-302, (1993)]