A diversified library of bacterial and fungal bifunctional cytochrome P450 enzymes for drug metabolite synthesis

…, M Schittmayer, S Kambourakis, M Vink…

Index: Weis, Roland; Winkler, Margit; Schittmayer, Matthias; Kambourakis, Spiros; Vink, Mandy; David Rozzell; Glieder, Anton Advanced Synthesis and Catalysis, 2009 , vol. 351, # 13 p. 2140 - 2146

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Citation Number: 32

Abstract

Abstract Innovative biohydroxylation catalysts for the preparation of drug metabolites were developed from scratch. A set of bacterial and fungal sequences of putative and already known bifunctional P450 enzymes was identified by protein sequence alignments, expressed in Escherichia coli and characterised. Notably, a fungal self-sufficient cytochrome P450 (CYP) from Aspergillus fumigatus turned out to be especially stable during catalyst ...