Autophagy 2005-04-01

Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae.

Taras Y Nazarko, Ju Huang, Jean-Marc Nicaud, Daniel J Klionsky, Andrei A Sibirny

Index: Autophagy 1(1) , 37-45, (2005)

Full Text: HTML

Abstract

Yarrowia lipolytica was recently introduced as a new model organism to study peroxisome degradation in yeasts. Transfer of Y. lipolytica cells from oleate/ethylamine to glucose/ammonium chloride medium leads to selective macroautophagy of peroxisomes. To decipher the molecular mechanisms of macropexophagy we isolated mutants of Y. lipolytica defective in the inactivation of peroxisomal enzymes under pexophagy conditions. Through this analysis we identified the gene YlTRS85, the ortholog of Saccharomyces cerevisiae TRS85 that encodes the 85 kDa subunit of transport protein particle (TRAPP). A parallel genetic screen in S. cerevisiae also identified the trs85 mutant. Here, we report that Trs85 is required for nonspecific autophagy, pexophagy and the cytoplasm to vacuole targeting pathway in both yeasts.

Related Compounds

Related Articles:

Lack of involvement of CEP adducts in TLR activation and in angiogenesis.

2014-01-01

[PLoS ONE 9(10) , e111472, (2014)]

Human hAtg2A protein expressed in yeast is recruited to preautophagosomal structure but does not complement autophagy defects of atg2Δ strain.

2011-01-01

[Acta Biochim. Pol. 58(3) , 365-74, (2011)]

Yeast Mon2p is a highly conserved protein that functions in the cytoplasm-to-vacuole transport pathway and is required for Golgi homeostasis.

2005-10-15

[J. Cell Sci. 118(Pt 20) , 4751-64, (2005)]

Atg19p ubiquitination and the cytoplasm to vacuole trafficking pathway in yeast.

2005-11-25

[J. Biol. Chem. 280(47) , 39067-76, (2005)]

Yeast aminopeptidase I. Chemical composition and catalytic properties.

1976-05-13

[Biochim. Biophys. Acta 429(3) , 933-49, (1976)]

More Articles...