Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography.

Xiao Wang, Chuange Cheng, Qinglei Sun, Fuwei Li, Jianhua Liu, Chengchao Zheng

Index: J. Chromatogr. A. 1075 , 127-131, (2005)

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Abstract

Four flavonoids, apigenin-7-O-neohesperidoside, luteolin-7-O-glucoside, apigenin-7-O-glucoside and kaempferol-7-O-glucoside have been isolated and purified for the first time from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography with a two-phase solvent system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v). Then, 5 mg apigenin-7-O-neohesperidoside, 4 mg luteolin-7-O-glucoside, 9 mg apigenin-7-O-glucoside and 2.5 mg kaempferol-7-O-glucoside could be obtained after injecting 40 mg sample and their purities were 94, 97, 97 and 96%, respectively. All these constituents were identified by mass spectrometry and nuclear magnetic resonance.