Monitoring of Leuconostoc population during sauerkraut fermentation by quantitative real-time polymerase chain reaction.

So-Young Kim, Ki-Seon Yoo, Yujin Kim, Eunyoung Seo, Beom Soo Kim, Nam Soo Han

Index: J. Microbiol. Biotechnol. 21(10) , 1069-72, (2011)

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Abstract

A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).