Unstable propoxyphene metabolite excreted in human urine is detected by liquid chromatography-tandem mass spectrometry.

Bridgit Crews, Charles Mikel, Sergey Latyshev, Robert West, Amadeo Pesce

Index: J. Anal. Toxicol. 33(7) , 379-83, (2009)

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Abstract

A dilute and shoot method for measuring norpropoxyphene in human urine using liquid chromatography-tandem mass spectrometry distinguishes two different metabolites of propoxyphene, norpropoxyphene (m/z 326) and a dehydrated rearrangement product (m/z 308). The metabolite formed from the rearrangement and dehydration of norpropoxyphene is excreted in human urine and also may be formed from the chemical degradation of norpropoxyphene. Previously, these two metabolites were indistinguishable by gas chromatography- mass spectrometry methods that use an alkaline extraction that converts norpropoxyphene into its rearrangement product. The degradation of norpropoxyphene presents a challenge for its analytical quantitation, and methods for circumventing these issues are presented.