Selective cross-linking of interacting proteins using self-labeling tags

…, G Lukinavicius, KT Tan, K Johnsson

Index: Brun, Matthias A.; Tan, Kui-Thong; Nakata, Eiji; Hinner, Marlon J.; Johnsson, Kai Journal of the American Chemical Society, 2009 , vol. 131, p. 5873 - 5884

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Citation Number: 47

Abstract

We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative interacting proteins as fusion to CLIP-tag or SNAP-tag, two protein tags that can be specifically labeled with synthetic probes. Bifunctional molecules that contain the ...