High-throughput log P determination by ultraperformance liquid chromatography: a convenient tool for medicinal chemists.

Yveline Henchoz, Davy Guillarme, Serge Rudaz, Jean-Luc Veuthey, Pierre-Alain Carrupt

Index: J. Med. Chem. 51 , 396-9, (2008)

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Abstract

Accurate determinations of lipophilicity indices benefit from recent advances in chromatographic sciences such as the launch of ultraperformance liquid chromatography (UPLC). The fast strategy presented here emerges as a powerful method suitable for high-throughput log P measurements of therapeutic compounds in isocratic and gradient modes. Because UPLC columns are highly stable in basic pH conditions, this approach allows a direct lipophilicity estimation of basic compounds in their neutral forms.