Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos.

Jeffrey R Sommer, E Bruce Collins, Jose L Estrada, Robert M Petters

Index: Anim. Reprod. Sci. 100(3-4) , 402-10, (2007)

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Abstract

An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse, PG600 and Chorulon along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal.