Radio-detection high-performance liquid chromatographic enzyme assay for inhibitors of fungal sterol delta 14-reductase.

C C Steel

Index: J. Chromatogr. A. 566(2) , 435-43, (1991)

Full Text: HTML

Abstract

An enzyme assay for inhibitors of fungal sterol delta 14-reductase employing isocratic reversed-phase high-performance liquid chromatography is described. A Hypersil 5-microns octadecylsilyl (ODS) column (250 mm x 4.6 mm I.D.) was used and a mobile phase consisting of methanol-water-ethanol (86:4:10, v/v) was pumped at a flow-rate of 1.5 ml/min. Typical analysis times were 15 min. Using [4-14C]ignosterol as a substrate and an enzyme preparation from Saccharomyces cerevisiae, this method was used to compare the inhibition of sterol delta 14-reductase by the fungicides fenpropidin and fenpropimorph with three N-substituted 8-azadecaline compounds.


Related Compounds

Related Articles:

Investigations into the source of two fungicides measured in the air for 24 hours following application to a cereal crop.

2009-01-01

[Commun. Agric. Appl. Biol. Sci. 74(1) , 37-46, (2009)]

Inhibition of microbial cholesterol oxidases by dimethylmorpholines.

1990-01-01

[J. Steroid Biochem. 35(1) , 107-13, (1990)]

A sterol C-14 reductase encoded by FgERG24B is responsible for the intrinsic resistance of Fusarium graminearum to amine fungicides.

2011-06-01

[Microbiology 157(Pt 6) , 1665-75, (2011)]

Molar absorptivities of 2,4-D, cymoxanil, fenpropidin, isoproturon and pyrimethanil in aqueous solution in the near-UV.

2006-01-01

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 63(1) , 103-10, (2006)]

Pesticide cocktails can interact synergistically on aquatic crustaceans.

2010-05-01

[Environ. Sci. Pollut. Res. Int. 17(4) , 957-67, (2010)]

More Articles...