Preparation and properties of gelatin-immobilized beta-glucosidase from Pyrococcus furiosus.

Hidetaka Nagatomo, Yoh-ichi Matsushita, Kazuhiro Sugamoto, Takanao Matsui

Index: Biosci. Biotechnol. Biochem. 69(1) , 128-36, (2005)

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Abstract

Hyperthermostable beta-glucosidase from Pyrococcus furiosus was enclosed in gelatin gel by cross-linking with transglutaminase. Gelatin-immobilized beta-glucosidase was considerably more thermostable than the native enzyme. Lyophilized immobilisate was stored at 90 degrees C for 1 month without loss of activity. The immobilized beta-glucosidase catalyzed transglucosylation of 5-phenylpentanol with 10.0 equivalent of cellobiose at pH 5.0 and 70 degrees C for 12 h to afford 5-phenylpentyl beta-D-glucopyranoside in 41% yield. The immobilized enzyme was more effective than the native one in transglucosylation. The gelatin-immobilized Pfu-beta-glucosidase recovered from the first run of the reaction was reusable on successive runs.