Drug quantitation on a benchtop liquid chromatography-tandem mass spectrometry system.

P R Tiller, J Cunniff, A P Land, J Schwartz, I Jardine, M Wakefield, L Lopez, J F Newton, R D Burton, B M Folk, D L Buhrman, P Price, D Wu

Index: J. Chromatogr. A. 771(1-2) , 119-25, (1997)

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Abstract

The specificity and selectivity of LC-MS-MS is illustrated to explain why LC-MS-MS has become the method of choice for quantitation within the pharmaceutical industry. Two assays are described that demonstrate the facility with which new ion trap technology can utilize the selectivity and sensitivity of LC-MS-MS to quantitate trace level components within complex matrices, in particular human plasma. One assay undergoes a validation procedure and demonstrates the utility of this new technology for drug quantitation within a regulated environment.