Chemical Communications 2011-08-28

Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy.

Doori Oh, Seungah Lee, Seong Ho Kang

Index: Chem. Commun. (Camb.) 47 , 9137-9139, (2011)

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Abstract

In a free solution of 10.0 mM Gly-Gly (pH 8.2), the flow directions of native-DNA and DNA molecules intercalated with the fluorescent dye YOYO-1 were reversed in the microchip channel. These results clearly showed that in a confined space and under specific environmental conditions, the fluorescent dye modified the original properties and behavior of the native-DNA molecule.

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