Drug Metabolism and Disposition

Small molecule quantification by liquid chromatography-mass spectrometry for metabolites of drugs and drug candidates

UP Dahal, JP Jones, JA Davis, DA Rock

Index: Dahal, Upendra P.; Jones, Jeffrey P.; Davis, John A.; Rock, Dan A. Drug Metabolism and Disposition, 2011 , vol. 39, # 12 p. 2355 - 2360

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Citation Number: 18

Abstract

Abstract Identification and quantification of the metabolites of drugs and drug candidates are routinely performed using liquid chromatography-mass spectrometry (LC-MS). The best practice is to generate a standard curve with the metabolite versus the internal standard. However, to avoid the difficulties in metabolite synthesis, standard curves are sometimes prepared using the substrate, assuming that the signal for substrate and the metabolite ...