Variation in mitochondrial NADH dehydrogenase subunit 5 and NADH dehydrogenase subunit 4 genes in the Chagas disease vector Triatoma infestans (Hemiptera: Reduviidae).

Cintia J Fernández, Alicia R Pérez de Rosas, Beatriz A García

Index: Am. J. Trop. Med. Hyg. 88(5) , 893-6, (2013)

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Abstract

Variation in mitochondrial NADH dehydrogenase subunit 5 (ND5) and NADH dehydrogenase subunit 4 (ND4) genes was surveyed in Triatoma infestans from 24 localities of Argentina. The DNA sequence comparisons of 2,183 basepairs of the mitochondrial genome, which include the complete sequence of ND5 (1,712 basepairs) and 401 basepairs of ND4 genes, showed 19 haplotypes determined by 48 variable sites and a nucleotide diversity value of 0.292%. Twenty-six (65%) substitutions were synonymous, and there were 14 (35%) predicted amino acid replacements in ND5. In ND4, 5 (62.5%) substitutions were synonymous and 3 (37.5%) were replacement sites. Samples from six localities studied shared one haplotype and the rest of the localities had different haplotypes. The amplified regions should be useful for population genetic studies.