Comparative study of biological activity of three commercial products of Sasa senanensis Rehder leaf extract.

Hiroshi Sakagami, Shoko Iwamoto, Tomohiko Matsuta, Kazue Satoh, Chiyako Shimada, Taisei Kanamoto, Shigemi Terakubo, Hideki Nakashima, Yurika Morita, Atsuko Ohkubo, Tadashi Tsuda, Katsuyoshi Sunaga, Madoka Kitajima, Hiroshi Oizumi, Takaaki Oizumi

Index: In Vivo 26(2) , 259-64, (2012)

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Abstract

We have previously reported that alkaline extract of Sasa senanensis leaves (SE) has several biological activities characteristic of lignin-carbohydrate complex (LCC). In the present study, we compared the biological activity of three commercially available products of SE (products A, B and C).Cell viability of mock-infected, HIV-infected, UV-irradiated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Radical intensity was determined by electron spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4.Product A is a pure SE that contains Fe(II)-chlorophyllin, whereas products B and C contain Cu(II)-chlorophyllin and less LCC. Product C is supplemented with ginseng and pine (Pinus densiflora) leaf extracts. Product A exhibited 5-fold higher anti-HIV, 4-fold higher anti-UV, 5-fold higher hydroxyl radical-scavenging, and 3-fold lower CYP3A4 inhibitory activities as compared to those of product B, and 5-fold higher, 1.5-fold higher, comparable, and 7-fold lower activities, respectively, as compared to those of product C.The present study demonstrates for the first time the superiority of product A over products B and C, suggesting the beneficial role of LCC and Fe(II)-chlorophyllin.