Inhibition of phosphatidylinositol kinase by toyocamycin.

H Nishioka, T Sawa, M Hamada, N Shimura, M Imoto, K Umezawa

Index: J. Antibiot. 43 , 1586-1589, (1990)

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Abstract

We have screened toyocamycin as an inhibitor of phosphatidylinositol kinase. It inhibited the enzyme of A431 cell membrane with an IC50 of 3.3 micrograms/ml. Adenosine and formycin A also inhibited the enzyme, but other 6 related nucleosides did not. Although orobol and 2,3-dihydroxybenzaldehyde that inhibit phosphatidylinositol kinase inhibited in situ phosphatidylinositol turnover, toyocamycin did not.