Journal - Association of Official Analytical Chemists 1985-01-01

Sensitive determination of ethopabate residues in chicken tissues by liquid chromatography with fluorometric detection.

T Nagata, M Saeki, H Nakazawa, M Fujita, E Takabatake

Index: J. Assoc. Off. Anal. Chem. 68(1) , 27-8, (1985)

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Abstract

A liquid chromatographic (LC) method is described for determination of ethopabate residues in chicken tissues. The drug is extracted from tissues with acetonitrile, and the extract is concentrated to 2-3 mL. This aqueous solution is rinsed with ethyl acetate and cleaned up by Florisil column chromatography. LC analysis is carried out on a Zorbax ODS column, and ethopabate is quantitated by using a fluorometric detector set at 306 nm (excitation) and 350 nm (emission). Recoveries of ethopabate added to chicken tissues at levels of 0.01 and 0.05 ppm were 87.8 and 92.7%, respectively. The detection limit was 100 pg for ethopabate standard, and 0.5 ppb in chicken tissues.


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