Oleyl-chitosan nanoparticles based on a dual probe for optical/MR imaging in vivo.

Chang-Moon Lee, Doorye Jang, Jin Kim, Su-Jin Cheong, Eun-Mi Kim, Min-Hee Jeong, Sun-Hee Kim, Dong Wook Kim, Seok Tae Lim, Myung-Hee Sohn, Yong Yeon Jeong, Hwan-Jeong Jeong

Index: Bioconjug. Chem. , 186-192, (2011)

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Abstract

Oleic acid-conjugated chitosan (oleyl-chitosan) is a powerful platform for encapsulating oleic acid-decorated iron oxide nanoparticles (ION), resulting in a good magnetic resonance imaging (MRI) probe. Oleyl-chitosan could self-assemble into core-shell structures in aqueous solution and provide the effective core compartment for loading ION. ION-loaded oleyl-chitosan nanoparticles showed good enhanced MRI sensitivity in a MR scanner. Cy5.5 dye was accessed to the oleyl-chitosan conjugate for near-infrared (NIR) in vivo optical imaging. After intravenous injection of ION-loaded Cy5.5-conjugated oleyl-chitosan (ION-Cy5.5-oleyl-chitosan) nanoparticles in tumor-bearing mice, both NIRF and MR imaging showed the detectable signal intensity and enhancement in tumor tissues via enhanced permeability and retention (EPR) effect. Tumor accumulation of the nanoparticles was confirmed through ex vivo fluorescence images and Prussian blue staining images in tumor tissues. It is concluded that ION-Cy5.5-oleyl-chitosan nanoparticle is highly an effective imaging probe for detecting tumor in vivo.