Determination of piracetam in rat plasma by LC-MS/MS and its application to pharmacokinetics.

Xianqin Wang, Jiayin Zhu, Renai Xu, Xuezhi Yang, Haiya Wu, Dan Lin, Faqing Ye, Lufeng Hu

Index: Biomed. Chromatogr. 24(10) , 1108-12, (2010)

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Abstract

A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of piracetam in rat plasma was developed and validated over the concentration range of 0.1-20 µg/mL. After addition of oxiracetam as internal standard, a simplified protein precipitation with trichloroacetic acid (5%) was employed for the sample preparation. Chromatographic separation was performed by a Zorbax SB-Aq column (150 × 2.1 mm, 3.5 µm). The mobile phase was acetonitrile-1% formic acid in water (10:90 v/v) delivered at a flow rate of 0.3 mL/min. The MS data acquisition was accomplished in multiple reaction monitoring mode with a positive electrospray ionization interface. The lower limit of quantification was 0.1 µg/mL. For inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 9%, and the accuracy was within the 94.6-103.2% range. The developed method was successfully applied to pharmacokinetic studies of piracetam in rats following single oral administration dose of 50 mg/kg.Copyright © 2010 John Wiley & Sons, Ltd.