European Biophysics Journal 2012-08-01

Thermodynamic interactions of a cis and trans benzanilide with Escherichia coli bacterial membranes.

Sarah R Dennison, Timothy J Snape, David A Phoenix

Index: Eur. Biophys. J. 41(8) , 687-93, (2012)

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Abstract

The activity of a membrane interactive cis and trans benzanilide against Escherichia coli membrane mimics was investigated using Langmuir monolayers. It was found that in the presence of E. coli lipid mix monolayers, cis-benzanilide induced maximal surface pressure changes of 1 mN m(-1), whereas a reduced interaction was observed with trans-benzanilide. Compression isotherm analysis of these monolayers showed ∆G(mix) < 0, indicating membrane stabilisation by both compounds. The binding coefficient for trans-benzanilide was circa 890 μM and for the cis-benzanilide was 820 μM, suggesting that both benzanilide compounds bound to the E. coli membranes.


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