Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology 1997-09-01

Effect of 3-amino-1-propanol on the phosphatidylinositol (PI) and glycosyl phosphatidylinositol (GPI) systems of Tetrahymena.

P Kovács, L Kõhidai, G Csaba

Index: Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 118(1) , 83-7, (1997)

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Abstract

3-Amino-1-propanol (AP), a substance replacing ethanolamine in phosphatidylethanolamine (PE) significantly reduced 32P incorporation to phosphatidylinositol (PI) and glycosyl-phosphatidylinositol (GPI) in the unicellular organism Tetrahymena pyriformis. At 10 mM, AP completely inhibited the incorporation of 32P into PI. 3H-arachidonate incorporation into PI was also inhibited, while that into diacylglycerol (DAG) was high. The experiments indicate the presence and metabolism of inositol phospholipids and GPI in T. pyriformis.

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