Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Shen Luo, Nancy B Wehr, Rodney L Levine
Index: Anal. Biochem. 350(2) , 233-8, (2006)
Full Text: HTML
Abstract
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to develop a rapid and sensitive method for detection and quantitation of proteins in gels and on blots. The fluorescence response is quantitative for protein content between 10 ng and 20 microg per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection.
Related Compounds
Related Articles:
2014-08-22
[Neurosci. Lett. 578 , 176-81, (2014)]
Differences in copper bioaccumulation and biological responses in three Mytilus species.
2015-03-01
[Aquat. Toxicol. 160 , 1-12, (2015)]
2011-01-01
[PLoS ONE 6(7) , e22801, (2011)]
Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity.
2009-10-14
[World J. Gastroenterol. 15(38) , 4799-805, (2009)]
Analysis and testing of biological stains--the Biological Stain Commission Procedures.
2002-01-01
[Biotech. Histochem. 77(5&6) , 237-275, (2002)]