[The disruptive effect of N'N-methylene-bis on the function of FRTL-5 cells].

Yong-Jie Qiu, Hao Zhang, Ding Sun, Yun-Liang Qiu, Jin-Tao Wang

Index: Sichuan Da Xue Xue Bao. Yi Xue Ban 41(1) , 73-6, (2010)

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Abstract

To study the impact of N' N-methylene-bis on thyroglobulin produced by FRTL-5 cells, and to explore the potential of using FRTL-5 cells to screen environmental thyroid hormone disruptors in vitro.The FRTL-5 cells were treated with 0.1, 1.0 and 10.0 microg/mL N'N-methylene-bis for 48 hours, respectively. The concentrations of thyroglobulin in the medium of the treated cells were detected by radioimmunoassay. The expression of thyroid peroxidases in the FRTL-5 cells was assessed by enzyme cytochemistry technique. The ultrastructure of the cells was also observed.The FRTL-5 cells treated with 0.1 and 1.0 microg/mL of N' N-methylene-bis produced less thyroglobulin than the controls (P < 0.05). No thyroglobulin was detected with the cells treated with 10.0 microg/mL of N' N-methylene-bis. No difference in the expression of thyroid peroxidases was found between the treated cells and the controls. The treated cells had expanded rough endoplasmic reticulum.N' N-methylene-bis disrupts the bio-function of thyroid by damaging the rough endoplasmic reticulum of thyroid follicular cells. FRTL-5 cells can be used for screening thyroid hormone disruptors in vitro.