In Vitro: Cellular and Developmental Biology 1985-05-01

A rapid naphthol yellow S method for measuring the cellular protein content of anchorage cultures.

P Skehan, S J Friedman

Index: In Vitro Cell. Dev. Biol. 21(5) , 288-90, (1985)

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Abstract

A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound dye is removed by a series of four 2.5 min washes in 1% acetic acid, and protein-bound dye extracted with 10 mM unbuffered Tris base for spectrophotometric optical density determination at 433 nm. The NYS method exhibited a least-squares correlation coefficient of 0.99997 with the Oyama-Eagle Lowry method.

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