Amine functional monodisperse microbeads via precipitation polymerization ofN-vinyl formamide: Immobilized laccase for benzidine based dyes degradation

Bunyamin Karagoz, Gulay Bayramoglu, Begum Altintas, Niyazi Bicak, M. Yakup Arica, Bunyamin Karagoz, Gulay Bayramoglu, Begum Altintas, Niyazi Bicak, M. Yakup Arica

Index: Bioresour. Technol. 102(13) , 6783-90, (2011)

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Abstract

Densely cross-linked poly(vinylamine) microbeads (∼2 μm) were prepared by precipitation copolymerization of N-vinyl formamide and ethylene glycoldimethacrylate in acetonitrile. The formamido groups of the microbeads were hydrolyzed into amino groups. Then, amino-functionalized microbeads were used for covalent immobilization of laccase via glutaraldehyde coupling. The average amount of immobilized enzyme was 18.7 mg/g microbeads. Kinetic parameters, V max and K m values were determined as 20.7 U/mg protein and 2.76 × 10 −2 mmol/L for free enzyme and 15.8 U/mg protein and 4.65 mmol/L for the immobilized laccase, respectively. The immobilized laccase was operated in a batch reactor for the degradation of two different benzidine based dyes (i.e., Direct Blue 1 and Direct Red 128). The laccase immobilized on the microbeads was very effective for removal of these dyes which interfere with the hormonal system.