Design and synthesis of novel photoaffinity probes for study of the target proteins of oleanolic acid.
Liying Zhang, Yingxia Zhang, Jizhe Dong, Jun Liu, Luyong Zhang, Hongbin Sun
Index: Bioorg. Med. Chem. Lett. 22 , 1036-9, (2012)
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Abstract
To explore the molecular mechanisms of oleanolic acid, two novel photoaffinity probes were synthesized based on the structure-activity relationship reported previously. Their potency were evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa), a known target protein of oleanolic acid. The inhibitory activity of probe 2 was only about two-fold less potent than the mother compound oleanolic acid. The photoaffinity labeling experiments were also performed and two proteins were specifically tagged by probe 2. The results suggest that the synthesized probes could be used as powerful tools to isolate and identify the target proteins of oleanolic acid.Copyright © 2011 Elsevier Ltd. All rights reserved.
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