Purification and structure determination of gelatinase and collagenase inhibitors from Viola patrinii fermentation extracts.

Kyoung-Sook Kim, Yeon-Joo Kwak, Kyung-Mi Kim, Hai Yang Yu, Byoung-Won Kang, Eunsook Chung, Young-Choon Lee, Jung-In Kim, Jai-Heon Lee

Index: Immunopharmacol. Immunotoxicol. 32(4) , 614-6, (2010)

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Abstract

Investigation of collagenase and gelatinase inhibitory natural components afforded two isoflavonoids. Two isoflavonoids, tectorigenin-7-O-β-D-glucoside (1) and luteolin-7-O-β-D-glucuronopyranoside (2), were isolated from ethyl acetate fraction of Viola patrinii fermentation extracts (VPFE). Of these, compounds 1 and 2 exhibited collagenase inhibitory activity (IC(50)) at a concentration of less than 1.5 μM, and compound 2 showed gelatinases A and B inhibitory activity (IC(50)) at 0.3 μM and 0.8 μM, respectively.