Polyphenolic compounds from callus cultures of Iris pseudacorus.

Darya V Tarbeeva, Sergey A Fedoreyev, Marina V Veselova, Anatoliy I Kalinovskiy, Ludmila D Seletskaya, Tamara I Mazurok, Victor P Bulgakov

Index: Nat. Prod. Commun. 8(10) , 1419-20, (2013)

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Abstract

A callus culture of Iris pseudacorus L. (Iridaceae) was established from plant leaves using a modified Murashige and Skoog medium. A derivative of cinnamic acid (lavandoside) (1), a neolignan (dehydrodiconiferyl alcohol-4-O-beta-D-glucopyranoside) (2) as well as three isoflavonoids, tectoridin (3), tectorigenin (4), and iristectorigenin A (5) were isolated from the callus culture. Under normal conditions, the calli accumulated 0.4% DW of polyphenols. The addition of phenylalanine to a concentration of 1 mM resulted in a 1.5-fold increase in isoflavonoid production, allowing the accumulation of 0.69% of polyphenols in the callus dry weight. Tectorigenin, a promising chemotherapeutic and chemopreventive agent for the treatment of carcinomas, was produced in I. pseudacorus calli in high quantities (0.3% DW).