Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3.

Kakuhei Isawa, Kenichi Hojo, Nobuo Yoda, Tomonori Kamiyama, Seiya Makino, Mizue Saito, Hitomi Sugano, Chinami Mizoguchi, Saori Kurama, Mika Shibasaki, Noriko Endo, Yoshio Sato

Index: Biosci. Biotechnol. Biochem. 66(3) , 679-81, (2002)

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Abstract

We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including 1H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.