Biochemical Society Transactions 1983-10-01

Inhibition of 2,3-oxidosqualene: beta-amyrin-cyclase, S-adenosyl-L-methionine: cycloartenol C-24-methyltransferase and cycloeucalenol: obtusifoliol isomerase by rationally designed molecules containing a tertiary amine function.

A Rahier, P Bouvier, L Cattel, A Narula, P Benveniste

Index: Biochem. Soc. Trans. 11(5) , 537-43, (1983)

Full Text: HTML

Abstract

25-Azacycloartanol (I), 2-aza-2-dihydrosqualene (II) and Tridemorph (2,6-dimethyl-N-tridecylmorpholine) (III) are potent inhibitors of higher plant sterol biosynthesis. The first two molecules have been designed using rational enzymological concepts. I, II and III were shown to inhibit the S-adenosyl-L-methionine: cycloartenol C-24-methyltransferase, the 2,3-oxidosqualene: beta-amyrin-cyclase and the cycloeucalenol: obtusifoliol isomerase, respectively. Inhibition was demonstrated either in vivo on bramble cell suspensions or in vitro on microsomes from maize seedlings. Each inhibitor has been shown to have a high affinity for its presumed enzymic target and only negligible inhibitory action on the other two enzymes. The applications of these results to further physiological studies are discussed.


Related Compounds

Related Articles:

Effect of some sterol-biosynthesis-inhibiting fungicides on the biosynthesis of polyisoprenoid compounds in barley seedings.

1989-01-01

[Steroids 53(3-5) , 393-412, (1989)]

[Teratogenic effect of the fungicide calixin].

1981-01-01

[Vopr. Pitan. (6) , 55-61, (1981)]

Human lamin B receptor exhibits sterol C14-reductase activity in Saccharomyces cerevisiae.

1998-06-15

[Biochim. Biophys. Acta 1392(2-3) , 233-44, (1998)]

Synthesis and structure revision of calyxin natural products.

2006-04-14

[J. Org. Chem. 71(8) , 3176-83, (2006)]

Inhibition of microbial cholesterol oxidases by dimethylmorpholines.

1990-01-01

[J. Steroid Biochem. 35(1) , 107-13, (1990)]

More Articles...