Journal of Immunological Methods 1993-08-09

The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.

R O'Kennedy, P Keating

Index: J. Immunol. Methods 163(2) , 225-31, (1993)

Full Text: HTML

Abstract

Radioactive iodine has been used as an antibody label in many immunoassays. The feasibility of using non-radioactive iodine as a label was investigated. A microassay for iodide based on the Sandell-Kolthoff reaction was optimised. It was used to detect iodine-labelled antibody and successfully applied to the detection of human IgG in serum samples. The performance of this assay system compared with enzyme-linked immunosorbent assay using HRP was evaluated.

Related Compounds

Related Articles:

Use of ceric ammonium sulphate and two dyes, methyl orange and indigo carmine, in the determination of lansoprazole in pharmaceuticals.

2007-06-01

[Acta. Pharm. 57(2) , 211-20, (2007)]

Exploring a benzyloxyaniline linker utilizing ceric ammonium nitrate (CAN) as a cleavage reagent: solid-phase synthesis of N-unsubstituted beta-lactams and secondary amides.

2001-01-11

[Org. Lett. 3(1) , 53-6, (2001)]

Automated measurement of urinary iodine with use of ultraviolet irradiation.

1995-04-01

[Clin. Chem. 41(4) , 581-5, (1995)]

Free radical scavenging potential of L-proline: evidence from in vitro assays.

2008-02-01

[Amino Acids 34(2) , 315-20, (2008)]

Preparation and characterization of nanoparticles bearing heparin or dextran covalently-linked to poly(methyl methacrylate).

1999-01-01

[J. Biomater. Sci. Polym. Ed. 10(1) , 47-62, (1999)]

More Articles...